Composition for TaqMan probe real-time fluorescent quantitative PCR (Polymerase Chain Reaction) detection

• Main Authors: LI JINGYI, HE TAO, JIANG SHENG, GONG LIXIN, DUAN WENFANG
• Format: Patent
• Language: Chinese; English
• Published: 12.08.2022
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention provides a composition of a reverse transcription primer, a downstream primer and a TaqMan probe sequence. The reverse transcription primer sequence comprises the TaqMan probe sequence and a PCR (Polymerase Chain Reaction) downstream primer sequence. The composition disclosed by the invention has universality, and can be used for detecting different genes only by designing an upstream primer according to a target gene and using the upstream primer together with the composition disclosed by the invention, so that the early-stage preparation work of an experiment and the experiment operation process are simplified, and the experiment cost is greatly reduced. 本发明提供了一种逆转录引物、下游引物和TaqMan探针序列的组合物，所述逆转录引物序列包括TaqMan探针序列和PCR下游引物序列。本发明组合物具有通用性，只需要根据目标基因设计上游引物，与本发明组合物一起使用即可用于不同的基因检测，简化了实验前期准备工作以及实验操作过程，大大降低了实验成本。