Bacillus subtilis engineering bacterium as well as construction method and application thereof

• Main Authors: XUE ZHENGLIAN, WANG ZHOU, HUANG JUNBAO, LIU YAN, TANG JUN, ZHOU MENGJIE, HU LIUXIU, HU WENSONG, WU JING, HUANG XILIN, LIU QINGTAO
• Format: Patent
• Language: Chinese; English
• Published: 09.08.2022
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention relates to the technical field of molecular biology and metabolic engineering, in particular to bacillus subtilis engineering bacteria and a construction method and application thereof, and the construction method comprises the following steps: step 1, constructing an expression strain BU12; step 2, constructing to obtain a promoter PD8; step 3, replacing natural promoters of cascade signal molecules PhrG and RapG in the expression strain BU12 with a constitutive promoter Phag, so as to construct a BC01 strain; 4, at least two promoters in promoters of the BC01 strain capB, C, A and racE are replaced with the promoter PD8, and the bacillus subtilis engineering bacteria are obtained through construction. The bacillus subtilis engineering bacteria are used for fermentation regulation culture, the finally obtained poly-gamma-glutamic acid and D-type glutamic acid monomers are high in content, and a solid foundation is laid for widening the market application of the poly-gamma-glutamic acid in the a