Chitin synthase 1 (CHS1) gene of Embryonidae and application of chitin synthase 1 (CHS1) gene in aspect of RNAi (Ribonucleic Acid Interfere)-mediated pest control

• Main Authors: GAO CONGFEN, RAO CONG, PEI XINGUO, WU SHUNFAN
• Format: Patent
• Language: Chinese; English
• Published: 05.08.2022
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES, AS HERBICIDES; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS; PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTSTHEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TRAPPING; VINEGAR; WINE

The invention discloses a growth and development related gene chitin synthase 1 (CHS1) of Eriomycorus cerinus and an application of the growth and development related gene chitin synthase 1 (CHS1). The research finds that the expression of the silent gene CHS1 can obviously inhibit the growth and development of the pennymphis lividipennis, and the pennymphis lividipennis can be efficiently prevented and treated. Meanwhile, dsCHS1 of a silent target gene CHS1 is also designed, and the dsCHS1 can be injected into insect bodies to achieve the purpose of preventing and treating the apis cerana. The invention provides a candidate gene with a good effect for a future pest control strategy mediated by RNA interference (RNAi) of bee bugs, and provides a theoretical basis for future development and application of dsRNA insecticides based on the target spot. 本发明公开了一种点蜂缘蝽生长发育相关基因chitin synthetase 1(CHS1)及其应用。本发明研究发现沉默基因CHS1的表达可以显著抑制点蜂缘蝽的生长发育，能够高效防治点蜂缘蝽。同时还设计了沉默靶标基因CHS1的dsCHS1，可通过注射进入虫体内达到防治点蜂缘蝽的目的。本发明为将来点蜂缘蝽RNA干扰(RNAi)介