Method for rapidly detecting titer of non-starch polysaccharide enzyme for feed
The invention relates to the field of feed compound enzyme quality evaluation, and particularly discloses a method for rapidly detecting the titer of non-starch polysaccharide enzyme for feed, which comprises the following steps: S1, drawing a standard curve: firstly drawing a standard curve of gluc...
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Main Authors | , , , |
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Format | Patent |
Language | Chinese English |
Published |
02.08.2022
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Subjects | |
Online Access | Get full text |
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Summary: | The invention relates to the field of feed compound enzyme quality evaluation, and particularly discloses a method for rapidly detecting the titer of non-starch polysaccharide enzyme for feed, which comprises the following steps: S1, drawing a standard curve: firstly drawing a standard curve of glucose absorbance value and glucose concentration; s2, feed starch removal: mixing the feed and water according to the weight ratio of 1: (2-5), then adding alpha-amylase to react for 3 hours, then adding saccharifying enzyme to react for 4 hours, standing for 2-3 hours, and centrifuging to take precipitate, so as to obtain the starch-removed feed; s3, an in-vitro digestion stage: performing in-vitro enzymolysis digestion on the destarched feed and a to-be-detected enzyme solution to obtain a to-be-detected sample, and determining absorbance of the to-be-detected sample and the blank sample; s4, determining the reducing sugar: calculating the content of the reducing sugar according to the standard curve in the S1 by u |
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Bibliography: | Application Number: CN202210257241 |