Method for rapidly detecting titer of non-starch polysaccharide enzyme for feed

• Main Authors: ZHOU LIANGJUAN, HUANG HUI, ZHAO BANGHONG, XIA PINGYU
• Format: Patent
• Language: Chinese; English
• Published: 02.08.2022
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; VINEGAR; WINE

The invention relates to the field of feed compound enzyme quality evaluation, and particularly discloses a method for rapidly detecting the titer of non-starch polysaccharide enzyme for feed, which comprises the following steps: S1, drawing a standard curve: firstly drawing a standard curve of glucose absorbance value and glucose concentration; s2, feed starch removal: mixing the feed and water according to the weight ratio of 1: (2-5), then adding alpha-amylase to react for 3 hours, then adding saccharifying enzyme to react for 4 hours, standing for 2-3 hours, and centrifuging to take precipitate, so as to obtain the starch-removed feed; s3, an in-vitro digestion stage: performing in-vitro enzymolysis digestion on the destarched feed and a to-be-detected enzyme solution to obtain a to-be-detected sample, and determining absorbance of the to-be-detected sample and the blank sample; s4, determining the reducing sugar: calculating the content of the reducing sugar according to the standard curve in the S1 by u