Biomolecule immobilization method and application thereof

• Main Authors: CHEN YAO, ZHANG XING, ZHOU QINGQIAN, HUANG HE
• Format: Patent
• Language: Chinese; English
• Published: 02.08.2022
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention relates to a biological immobilization technology, and discloses a biomolecule immobilization method and application thereof. The immobilization method of the biomolecules comprises the following steps: in the presence of a reaction solvent, contacting the biomolecules, a metal salt and a forming agent, wherein the biomolecules are cytochrome c and/or glucose oxidase, and the forming agent contains imidazole and/or imidazole derivatives. The invention also provides the immobilized biomolecule obtained by the immobilization method, and an application of the immobilized biomolecule in at least one of a biological detector, food production and preparation of a clinical injection. According to the method, the biomolecules can be rapidly immobilized, the stability of the biomolecules is improved, and the biological activity of the biomolecules is efficiently maintained. 本发明涉及生物固定化技术，公开了一种生物分子的固定化方法及其应用。该生物分子的固定化方法包括以下步骤：在反应溶剂存在的条件下，将生物分子、金属盐与成型剂进行接触；其中，所述生物分子为细胞色素c和/或葡萄糖氧化酶，所述成型剂含有咪唑和/或咪唑衍生物。本发明还提供了该