Methods of assessing wound healing potency of population of mesenchymal stem cells and related methods of selecting mesenchymal stem cells and identifying tissues as starting material for producing population of mesenchymal stem cells

• Main Author: PHAN TOAN THANG
• Format: Patent
• Language: Chinese; English
• Published: 29.07.2022
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PHYSICS; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; TESTING; VINEGAR; WINE

The present invention relates to a method of assessing wound healing efficacy of a population of mesenchymal stem cells (MSC). Furthermore, the present invention relates to methods of selecting MSCs for producing a population of stem cells under cGMP conditions, methods of selecting a population of MSCs for producing a population of stem cells for subsequent drug administration. Furthermore, the present invention relates to a method of selecting a population of MSCs for generating a main cell bank, as well as a method of identifying tissues suitable as starting material for generating a population of MSCs for pharmaceutical use. The method comprises determining the level of at least two proteins selected from the group consisting of Angiopoietin 1 (Ang-1), Transforming Growth Factor Beta (TGF-Beta), Vascular Endothelial Growth Factor (VEGF) and Hepatocyte Growth Factor (HGF) secreted into the culture medium by MSC in the culture medium. 本发明涉及评估间充质干细胞(MSC)群体的伤口愈合效力的方法。此外，本发明涉及选择用于在cGMP条件下产生干细胞群体的MSC的方法，选择用于产生用