Ultrahigh-concentration continuous ethanol fermentation production method of yeast cells

• Main Authors: TONG XINYI, HUO YULIANG, DAI XIANGCHENG, CUI QI, PAN SHUXIN, XUE CHUANG, ZHOU WENJING, CHENG CHI, JIN YANMING, WANG HUAN, LI XINQI
• Format: Patent
• Language: Chinese; English
• Published: 29.07.2022
• Subjects: ACYCLIC OR CARBOCYCLIC COMPOUNDS; BEER; BIOCHEMISTRY; CHEMISTRY; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PROCESSES USING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses a method for ultrahigh-concentration continuous ethanol fermentation production of yeast cells. The method comprises the following steps: culturing ethanol producing bacteria; preparing saccharified liquid of wheat and corn; carrying out ultrahigh-concentration continuous ethanol fermentation by using ethanol producing bacteria; and weakening fermentation parameter oscillation of the ethanol production strain in the continuous ethanol fermentation process by using product in-situ separation (a gas stripping method or a membrane separation method), and carrying out continuous production of stable ultrahigh-concentration ethanol. According to the method, on the premise of not increasing equipment investment, single-stage tank ultrahigh-concentration continuous ethanol fermentation is effectively achieved, the concentration of residual sugar is controlled to be lower than 1 g/L, the industrial production requirement is met, and a new technical support is provided for ultrahigh-concentrati