High-activity hyaluronic acid-polypeptide conjugate as well as preparation method and application thereof

• Main Authors: YIN XI, SUN CONGLI, XI YUE, WANG ENSI
• Format: Patent
• Language: Chinese; English
• Published: 29.07.2022
• Subjects: CHEMISTRY; COMPOSITIONS BASED THEREON; DERIVATIVES THEREOF; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; ORGANIC MACROMOLECULAR COMPOUNDS; POLYSACCHARIDES; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; SPECIFIC USE OF COSMETICS OR SIMILAR TOILETPREPARATIONS; THEIR PREPARATION OR CHEMICAL WORKING-UP

The invention discloses a high-activity hyaluronic acid-polypeptide conjugate as well as a preparation method and application thereof. The preparation method of the high-activity hyaluronic acid-polypeptide conjugate comprises the following steps: preparing aldehyde modified hyaluronic acid; the preparation method comprises the following steps: dissolving aldehyde modified hyaluronic acid in a buffer solution, adding auxin and sodium cyanoborohydride, and reacting; ethyl carbazate is added for a reaction; finally, the hyaluronic acid-polypeptide conjugate is obtained. According to the technical scheme, the auxin can be perfectly connected to the hyaluronic acid, and the binding rate is about 70%; the secondary structure of the auxin in the hyaluronic acid-polypeptide conjugate disclosed by the invention is not changed; and in a skin tissue test, the hyaluronic acid-polypeptide conjugate can be effectively transferred to a corium layer. 本发明公开一种高活性透明质酸-多肽缀合物及其制备方法和应用。其中，高活性透明质酸-多肽缀合物的制备方法为：制备醛修饰的透明质酸；将醛修饰的透明质酸溶