Porcine delta coronavirus virulent strain and application thereof in vaccine preparation

• Main Authors: FANGPU COUNTY, LU YOUXIN, PENG XUAN, MA JUN, ZHANG YUHAN, FANG LIURONG, ZHOU YANRONG, XIAO SHAOBO
• Format: Patent
• Language: Chinese; English
• Published: 26.07.2022
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; VINEGAR; WINE

The invention belongs to the technical field of animal virology and animal infectious diseases, and particularly relates to a porcine delta coronavirus virulent strain and application of the virulent strain in vaccine preparation. According to the invention, a strain of PDCoV is separated from small intestine contents of piglets with diarrhea clinically, the strain is named as porcine delta coronavirus DHeB1, the strain is preserved in the China Center for Type Culture Collection with the preservation number of CCTCC NO: V202217, and the PDCoV DHeB1 has higher proliferation titer, stronger pathogenicity and better immunogenicity, and can be used for preparing PDCoV inactivated vaccines. 本发明属于动物病毒学与动物传染病学技术领域，具体涉及一株猪δ冠状病毒强毒株和该毒株在疫苗制备中的应用。本发明从临床发生腹泻仔猪的小肠内容物中分离到一株PDCoV，命名为猪δ冠状病毒DHeB1株，将其保藏于中国典型培养物保藏中心，保藏编号为CCTCC NO:V202217，该PDCoV DHeB1具有更高的增殖滴度、更强的致病性和更好的免疫原性，可用于制备PDCoV灭活疫苗。