Application of miR-491 and miR-381 in expression regulation and control of bovine hypothalamus CART after transcription

• Main Authors: DU HAIYAN, CHENG JUNLI, LI PENGFEI
• Format: Patent
• Language: Chinese; English
• Published: 22.07.2022
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PHYSICS; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TESTING; VINEGAR; WINE

The invention discloses an application of miR-491 and miR-381 in expression regulation and control of bovine hypothalamus CART after transcription. The application comprises the following steps: comparative analysis of the inhibition effect of miRNAs on CART; detecting gene expression of the 293T cell, including extracting and detecting total RNA (Ribonucleic Acid) of the cell; carrying out mRNA reverse transcription; carrying out reverse transcription on the miRNA; designing a primer; carrying out fluorescent quantitative PCR (Polymerase Chain Reaction) detection; detecting CART protein expression of the 293T cell, wherein the CART protein expression comprises cell total protein extraction; determining the protein concentration by a BCA method; loading a sample; performing electrophoresis; transferring the film; performing sealing; incubating the primary antibody and the secondary antibody; developing is conducted; the regulation and control of miR-491 and miR-381 on mouse CART expression comprises the follo