Pyridine ring-containing aryl alkene nitrile carbazole reaction type fluorine ion fluorescent probe as well as preparation method and application of pyridine ring-containing aryl alkene nitrile carbazole reaction type fluorine ion fluorescent probe

• Main Authors: YUE YI, YAN LONGJIA, LI DAN, RAO NIAN, LIU LI
• Format: Patent
• Language: Chinese; English
• Published: 22.07.2022
• Subjects: ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAININGELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN,SULFUR, SELENIUM OR TELLURIUM; ADHESIVES; CHEMISTRY; DYES; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FORELSEWHERE; MEASURING; METALLURGY; MISCELLANEOUS APPLICATIONS OF MATERIALS; MISCELLANEOUS COMPOSITIONS; NATURAL RESINS; ORGANIC CHEMISTRY; PAINTS; PHYSICS; POLISHES; TESTING

The invention relates to the technical field of detection, in particular to an aryl alkene nitrile carbazole reaction type fluorine ion fluorescent probe containing a pyridine ring as well as a preparation method and application of the aryl alkene nitrile carbazole reaction type fluorine ion fluorescent probe. According to the invention, carbazole is used as a fluorophore, a silicon-oxygen bond is used as a recognition group, a pyridine group is introduced through a Knoevenagel condensation reaction, and the compound 3-((2-((tert-butyldimethylsilyl) oxy)-9-propyl-9H-carbazole-3-yl) 2-(pyridine-2-yl) acrylonitrile is synthesized. The fluorine ions induce Si-O bond breakage through Lewis acid-base reaction to form O-attacking ortho-aryl allylazonitrile groups to perform cyclization, and the conjugate area of the probe is greatly increased, so that the quantum yield is greatly improved, and the fluorescence intensity is remarkably changed to realize the detection of the fluorine ions in a biological sample. The