Preparation method of rotavirus inactivated vaccine

• Main Authors: TIAN DAYONG, AN QI, ZHAO YUYING, ZHANG YACHUN
• Format: Patent
• Language: Chinese; English
• Published: 08.07.2022
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; VINEGAR; WINE

The invention relates to a preparation method of a rotavirus inactivated vaccine, and belongs to the technical field of biological medicines. The invention provides a preparation method of a rotavirus inactivated vaccine, the preparation method comprises the steps of virus release, virus clarification, virus inactivation, host DNA degradation, virus concentration and virus purification, the virus release step adopts a pressure crushing method to crush host cells, the virus clarification step adopts continuous flow centrifugation, and the virus purification step adopts continuous flow centrifugation to purify the host cells. The virus purification step adopts continuous flow sucrose density gradient centrifugation; according to the preparation method, a pressure crushing method is adopted to crush host cells in the virus release step, compared with freeze thawing crushing, the pressure crushing step is simple, continuous sample injection can be achieved, the operation time is greatly shortened, compared with u