Aptamer biosensor for detecting salmonella as well as preparation method and application of aptamer biosensor

• Main Authors: MAN CHAOXIN, JIANG YUJUN, YANG XINYAN, PANG LIDONG
• Format: Patent
• Language: Chinese; English
• Published: 05.07.2022
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PHYSICS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TESTING; VINEGAR; WINE

The invention discloses an aptamer biosensor for detecting salmonella as well as a preparation method and application of the aptamer biosensor, and belongs to the technical field of food safety. In order to solve the problems existing in salmonella detection in the prior art, the invention provides an aptamer biosensor, the detection principle of the aptamer biosensor is that a sandwich structure SMBs-Apt2-AuNPs fluorescence quenching system established by an SMBs-cDNA2 compound, an aptamer Apt2 and an AuNPs-cDNA3 probe can keep integrity in the absence of salmonella, and FRET is carried out on AuNPs and AgNCs in the system to quench fluorescence of the AgNCs; under the condition that the salmonella exists, the salmonella is combined with the Apt2 serving as a connecting bridge, the SMBs-Apt2-AuNPs system is destroyed, and the AuNPs-cDNA3 probe suspended in supernate and the salmonella combined with the Apt2 are removed through magnetic separation, so that fluorescence is not quenched. The aptamer biosensor o