Molecular marker SSR primer for identifying genetic relationship and variety of rehmannia glutinosa variety, kit and application

• Main Authors: YANG JINXING, LIU BIN, ZHANG YINGTAO, ZHANG YONGZHAN, CHEN GUOCAN, YANG TIEGANG, LI CHUNXIN, LIU YONGKANG, WANG YAN
• Format: Patent
• Language: Chinese; English
• Published: 05.07.2022
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention relates to the technical field of molecular biology and genetic breeding, in particular to a molecular marker SSR primer for genetic relationship and variety identification of rehmannia glutinosa varieties, a kit and application. The 50 pairs of SSR primers provided by the invention are used for respectively amplifying DNA of 17 planting resources in the Huanghuai region, and the derivative relationship, genetic difference, evolution relationship and the like among different varieties are determined through clustering analysis and genetic similarity analysis, so that a scientific basis can be provided for breeding and breeding. Meanwhile, by adopting the 15 pairs of SSR primers provided by the invention, different rehmannia varieties can be accurately distinguished through difference combination of amplification products among different primer pairs, so that the rehmannia varieties in 17 Huanghuai production areas can be distinguished and identified on the DNA level, and the SSR fingerprint spec