Starting culture medium for directly inducing field petioles of Chinese horse chestnut to regenerate adventitious buds and application of starting culture medium

• Main Authors: DONG YUANFU, WANG CUIYAN, LI HONGJING, FU HONGYING, MAO XIN, WANG JING, ZHANG YUANSHUAI, LI YULING, CHEN JUNQIANG, WANG JINNA, MAO XIUHONG, MA ANBAO
• Format: Patent
• Language: Chinese; English
• Published: 05.07.2022
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; NEW PLANTS OR PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES; TRAPPING

The invention discloses a starting culture medium for directly inducing field petioles of Chinese aesculus chinensis to regenerate adventitious buds and application of the starting culture medium. The starting culture medium is formed by adding 0.05-5.0 mg/L of compound sodium nitrophenolate, 0.05-3.0 mg/L of gibberellin (GA), 0.5-10.0 mg/L of 6-benzylamino adenine (6-BA), 0.1-1.0 mg/L of kinetin (KT), 0.05-5.0 mg/L of naphthylacetic acid (NAA), 30-50 g/L of cane sugar, 0.5-1.0 g/L of activated carbon and 4.0-6.0 g/L of agar into an MS culture medium. According to the culture medium, multiple plant growth regulators are combined, and adventitious buds are directly induced and regenerated by explants of field petioles of Aesculus sinensis. Technical support is provided for factory production of the excellent germplasm resources of the Chinese aesculus chinensis, and the problem that the excellent germplasm resources of the Chinese aesculus chinensis are short of supply is solved. 本发明公开了一种直接诱导中华七叶树大田叶柄再生出不定芽的启动