Method for coupling acridinium ester with antibody and application of method

• Main Author: ZHANG JIEXUAN
• Format: Patent
• Language: Chinese; English
• Published: 01.07.2022
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention discloses a method for coupling acridinium ester with an antibody and application of the method, and relates to the technical field of immunoassay, the method comprises the following steps: before the antibody is coupled with the acridinium ester, the antibody and EDTA disodium are subjected to a mixed reaction, compared with the prior art, the antibody treated by the EDTA disodium is coupled with the acridinium ester and is detected, and the detection sensitivity is high. The false positive phenomenon in chemiluminescence immunodetection can be effectively avoided or reduced, and the detection specificity is improved. 本发明公开了一种吖啶酯偶联抗体的方法及其应用，涉及免疫分析技术领域，该方法包括在抗体偶联吖啶酯之前，将抗体与EDTA二钠混合反应，与现有技术相比，对采用EDTA二钠处理后的抗体与吖啶酯偶联并进行检测，能有效地避免或减少化学发光免疫检测中出现的假阳性现象，提高检测的特异性。