Chip, method for preparing chip and application of chip

• Main Authors: HE CHENFEI, LIN YUBIN, SHEN YUE, JIANG XIANG'ER, YUAN CHAOFENG, WANG YONG
• Format: Patent
• Language: Chinese; English
• Published: 01.07.2022
• Subjects: CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE; PERFORMING OPERATIONS; PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL; TRANSPORTING

The invention provides a chip which comprises a substrate, the substrate is connected with a connecting molecule, one end, far away from the substrate, of the connecting molecule carries a hydroxyl group, the hydroxyl group is protected by an acid unstable group, the connecting molecule is connected with the substrate through an initial molecule and a spacer molecule, the starting molecule is connected with the substrate, the spacer molecule and the starting molecule form covalent linkage, one end of the connecting molecule is connected with one end, far away from the starting molecule, of the spacer molecule, and the spacer molecule comprises at least one monomer selected from amino heptanoic acid, succinic anhydride-hexamethylenediamine and TESHBA. The modified molecules on the surface of the chip are uniformly distributed, the requirement of subsequent biomacromolecule synthesis can be ensured, and the preparation method of the chip is simple, short in steps and beneficial to large-scale production. 本发明提出了