Sucrose phosphorylase capable of realizing extracellular secretory expression as well as preparation method and application of sucrose phosphorylase

• Main Authors: XIE RUFEI, FAN ZILING, XING ZHIWEI, LIANG HENGYU, HAN CHAO, TAN WEI, ZHANG HAO, CHEN MINLIANG, ZHOU PENG
• Format: Patent
• Language: Chinese; English
• Published: 28.06.2022
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses sucrose phosphorylase capable of realizing extracellular secretory expression as well as a preparation method and application thereof, and belongs to the technical field of enzyme engineering. According to the invention, a sucrose phosphorylase gene BaSP from animal bifidobacterium lactis subsp. Lactis is connected with a signal peptide N20, a recombinant expression plasmid pET-N20-BaSP is constructed, and escherichia coli BL21 (DE3) is transformed, so that the efficient induced extracellular secretion expression of the BaSP in the escherichia coli is realized. 本发明公开了一种可胞外分泌表达的蔗糖磷酸化酶及其制备方法和应用，属于酶工程技术领域。本发明将来源于动物双歧杆菌乳亚种(Bifidobacteriumanimalissubsp.lactis)的蔗糖磷酸化酶基因BaSP与一种信号肽N20连接，构建重组表达质粒pET-N20-BaSP并转化大肠杆菌BL21(DE3)，实现了BaSP在大肠杆菌中的高效诱导胞外分泌表达。