Preparation method and application of nano drug delivery system for inhibiting melanoma growth

• Main Authors: ZHENG ZHICHANG, CAO YONG, LI LIN, FU YUYIN, LI MING, FAN XINGHUA, YANG CHENGLI, BAI YANG, WEN ZHIPENG
• Format: Patent
• Language: Chinese; English
• Published: 28.06.2022
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; VINEGAR; WINE

The invention relates to the technical field of biological medicine, and discloses a preparation method of a nano drug delivery system for inhibiting melanoma growth, and the preparation method comprises the following steps: constructing shRNA-Ptpn2 plasmid, synthesizing HA-DOX, preparing an M1 macrophage membrane, and preparing a nano drug delivery system M1HD-coated RPR; the invention further discloses a nano drug delivery system and application. Targeted delivery of the drug in the tumor site in vivo is achieved, the drug is used for inhibition treatment of primary melanoma and pulmonary metastasis tumor, and a new breakthrough direction is provided for the dilemma that no significant and effective treatment scheme for melanoma exists at present; according to the present invention, shRNA is cut and converted into siRNA in the cell so as to provide the function of RNAi to silence the gene, the shRNA is adopted as the small interfering RNA sequence, and is cloned into the plasmid vector as the short hairpin