Method for simultaneously determining residues of three methoprene juvenile hormone analogues in tea leaves

• Main Authors: SUN DENGFENG, TANG XIANGKAI, SUN YUJIE, TIAN WEI, CHEN LU, FENG DEJIAN
• Format: Patent
• Language: Chinese; English
• Published: 14.06.2022
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention relates to a method for simultaneously determining residues of three methoprene juvenile hormone analogues in tea leaves, and belongs to the technical field of pesticide detection. The method for simultaneously determining the residues of the three methoprene juvenile hormone analogues in the tea comprises the following steps: preparing a sample to be detected: a, soaking the tea to be detected in water, adding 1% acetic acid-acetonitrile, anhydrous magnesium sulfate, sodium acetate and ceramic homogeneous protons, uniformly oscillating, and centrifuging at the speed of 4200r/min for 5 minutes at the temperature of 8 DEG C to obtain supernate 1; b, uniformly mixing the supernate 1 with anhydrous magnesium sulfate, PSA, C18 and GCB, and centrifuging at the speed of 4200r/min for 5 minutes at the temperature of 8 DEG C to obtain supernate 2; the method provided by the invention not only controls the influence of the matrix effect, but also ensures the recovery rate of the three juvenile hormone an