Method for detecting methyl mercury based on gold nano-enzyme peroxidase

• Main Authors: YANG YALING, YIN MENGJIA, YANG DEZHI, LI QIULAN, WEI DAQIAO
• Format: Patent
• Language: Chinese; English
• Published: 07.06.2022
• Subjects: CASTING; CHEMISTRY; COMPOUNDS THEREOF; INORGANIC CHEMISTRY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MAKING METALLIC POWDER; MANUFACTURE OF ARTICLES FROM METALLIC POWDER; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MEASURING; METALLURGY; NANOTECHNOLOGY; NON-METALLIC ELEMENTS; PERFORMING OPERATIONS; PHYSICS; POWDER METALLURGY; SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; TESTING; TRANSPORTING; WORKING METALLIC POWDER

According to the method, serotonin carbon dots and glucose are used as a reducing agent and a stabilizing agent to prepare carbon dot gold nano-enzyme, the carbon dot gold nano-enzyme has the characteristics and reducibility of quasi-peroxidase nano-enzyme and can catalyze reduction of methyl mercury, and due to the fact that the absolute electronegativity of Hg is smaller than that of Au, the carbon dot gold nano-enzyme can catalyze reduction of methyl mercury. Electrons transferred from Au-Hg amalgam are much easier than electrons transferred from Au, so that the catalytic activity of the nano-enzyme is enhanced after methyl mercury is introduced, TMB can be quickly oxidized into a blue oxidation product oxTMB by H2O2, on the basis of the principle, methyl mercury is quickly detected, a test strip capable of being used for on-site quick detection can be prepared, detection of the test strip is completed within 10 minutes, and the detection efficiency is greatly improved. The limit of quantitation of methyl