Molecular marker of wheat gliadin content related genetic locus

• Main Authors: LEI ZHENSHENG, GUAN HUIYUE, WU ZHENGQING, YANG PAN, WANG YAHUAN, LIU CONGCONG, ZHOU ZHENGFU
• Format: Patent
• Language: Chinese; English
• Published: 07.06.2022
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention belongs to the field of wheat molecular breeding, and particularly relates to a genetic marker related to the content of prolamin and components thereof in wheat grains. The inventor detects a major QTL related to the total prolamin content and the content of each component of prolamin alpha, gamma and omega at 32.8-38.5 Mb of a 6AS chromosome, and the phenotypic variation of 5.07-9.16% can be explained. After further positioning analysis, the genetic marker is located on a short arm of a 6A chromosome, and the 36th basic group of the nucleotide sequence of the genetic marker has mutation of a G/T allele; when the 36th basic group is G, the genotype is a GG genotype with high alcohol-soluble protein content, and when the 36th basic group is T, the genotype is a TT genotype with low alcohol-soluble protein content. By utilizing the genetic marker AX-108761918 (37.44 Mb), a wheat variety with high alcohol-soluble protein content and a genotype of GG can be effectively screened. 本发明属于小麦分子育种领域，具体涉及一