Fusion protein for catalyzing glucose to synthesize D-psicose and construction method thereof

• Main Authors: ZHU LIJUAN, ALAMU, XIONG WENLONG, YING HANJIE, JIA QING, ZHANG HUI, WANG SHIYUAN, LV YONGKUN, QU LINGBO, XU JINGLIANG, ZHAO ANQI
• Format: Patent
• Language: Chinese; English
• Published: 07.06.2022
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention provides a fusion protein for catalyzing glucose to synthesize D-psicose and a construction method of the fusion protein, and relates to the field of biochemical engineering. In order to avoid the problems of low conversion efficiency and the like caused by diffusion of an intermediate product in a double-enzyme catalysis system, the fusion protein connected by connecting peptides with different lengths is constructed, and the conversion efficiency is improved by constructing a substrate channel. Specifically, a glucose isomerase gene and D-psicose 3-epimerase are connected by using a flexible connecting peptide and a rigid connecting peptide with different lengths, and a result shows that the synthesis efficiency of D psicose is improved along with the increase of the length of the connecting peptide, and the rigid connecting peptide is superior to the flexible connecting peptide. According to the fusion protein provided by the invention, an intermediate product is transferred to a next enzyme