Suspension domestication method of HEK293T cells and application of suspension domestication method in lentivirus production

• Main Authors: JIANG JULING, ZHOU WENTING, WU SHANSHAN, CHEN ZEJIAN, XIONG ZICHEN
• Format: Patent
• Language: Chinese; English
• Published: 07.06.2022
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention provides a suspension domestication method of HEK293T cells and application of the suspension domestication method in lentivirus production, and aims to avoid the use of unknown culture medium components and animal-derived production materials in the culture process of lentivirus production cells, the suspension domestication method is simple and convenient in flow, short in domestication time and lower in cost; the adopted serum-free culture medium is clear in component and controllable in safety risk, process impurities are reduced, and quality control is relatively simple; the production process is easy to amplify, and the manpower consumption is low; an original cell bank (PCB), a main cell bank (MCB) and a working cell bank (WCB) of the suspension cells are established by the suspension cells obtained through amplification culture and domestication, the working cell bank (WCB) of the suspension system is applied to preparation and production of the lentivirus, and the yield of crude venom o