Method for rapidly detecting content of sufentanil in human saliva

• Main Authors: ZHENG ZIYANG, YAO SEN, SHI QIAOCUI, ZENG WENFANG, FU JIAYU, MO FAN, ZHOU YUCHANG, FAN JICAI
• Format: Patent
• Language: Chinese; English
• Published: 03.06.2022
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention relates to the field of drug detection, and discloses a method for rapidly detecting the content of sufentanil in human saliva, which comprises the following steps: (1) selecting qualitative ions and quantitative ions; (2) constructing a standard curve; (3) pretreatment of a saliva sample: taking the saliva sample, adding an internal standard, adding alkali liquor to adjust the pH value, adding anhydrous magnesium sulfate and ethyl acetate, centrifuging, and adding methanol; and (4) measuring the content of sufentanil in the to-be-measured sample liquid by pulse direct current spray ion source mass spectrometry. Compared with the prior art, the invention develops a simple pretreatment method for mass spectrometric detection in a saliva sample, can effectively eliminate interference of impurities in saliva and improve the detection sensitivity and stability, and is suitable for rapid detection of the sample; the complete method for detecting the content of sufentanil in the saliva sample through