Template-free enzymatic polynucleotide synthesis using photo-cleavable ligation

• Main Author: HOGAN ADRIAN
• Format: Patent
• Language: Chinese; English
• Published: 27.05.2022
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; VINEGAR; WINE

The present invention relates to methods and kits for template-free enzymatic synthesis of polynucleotides using light cleavable ligation. In some embodiments, such methods include the use of 3 '-O-NH2-dNTP monomers that can react with photocleavable products having free ketones to allow for synthesis and purification on the same or added support. 本发明涉及用于采用光可切割连接的无模板酶促合成多核苷酸的方法和试剂盒。在一些实施方案中，此类方法包括使用可与具有游离酮的光可切割产物反应的3'-O-NH2-dNTP单体，以允许在同一或添加的支撑物上进行合成和纯化。