Method for detecting antibiotic heterogeneous drug resistance and stability of blue-green algae

• Main Authors: YAN HANLU, ZHANG JIANYUN, HUANG YU, WANG ZHIYUAN, LIN YUQING, ZU YUNXIA, CHEN QIUWEN, CHEN CHENG, CHEN YUCHEN, CHEN XIAOXUE, HONG YIMING
• Format: Patent
• Language: Chinese; English
• Published: 27.05.2022
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROCESSES USING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses a method for detecting antibiotic heterogeneous drug resistance and stability of blue-green algae, and the method comprises the following steps: respectively culturing target species samples by adopting antibiotics with gradient concentrations, and respectively measuring the lowest antibiotic inhibition concentration of each target species sample by a microdilution method; taking the minimum value as the community MIC of the target species; antibiotics with the concentration being 0, 1 and n times that of the community MIC are taken to culture the to-be-detected blue-green algae respectively, n is larger than or equal to 2 and smaller than or equal to 8, and the number of cells of each group of blue-green algae is counted and marked as P0, P1 and Pn respectively; and if Pn is less than (10%-50%) P0, calculating the antibiotic heterogeneous drug resistance level f of the to-be-detected blue-green algae through the following formula: the false negative misjudgment probability of a blue-g