Method for inducing cyclocarya paliurus stem tissue culture germination by bacteriostatic agent

• Main Authors: MOU GUITING, WU XIANLIANG, LUO WENMIN, LIU YINGYING, ZHANG ZHENMING, WU GAOYIN
• Format: Patent
• Language: Chinese; English
• Published: 27.05.2022
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; NEW PLANTS OR PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES; TRAPPING

The invention discloses a method for tissue culture germination induction of a cyclocarya paliurus stem by using a bacteriostatic agent. The method comprises the following steps: acquiring the cyclocarya paliurus stem, pretreating the stem, disinfecting the stem, performing germination induction on the stem in different collection months, performing germination induction on the stem by using a plant growth regulator, and influencing the bacteriostatic effect and germination induction of a plant fungicide on the stem. The acquisition test material of the stem segment of the Cyclocarya paliurus is from the young stem segment of the Cyclocarya paliurus of five years old in the nursery field of the Institute of Biological Research of Guizhou Province; in the stem section pretreatment, stem sections (terminal buds are discarded, and 2-5 sections below the terminal buds) which are flourishing and free of plant diseases and insect pests in different months are collected, each section is cut to be about 10 cm long, a