Fusion gene, recombinant novel coronavirus high-efficiency immune tripod molecular DNA vaccine, and construction method and application of recombinant novel coronavirus high-efficiency immune tripod molecular DNA vaccine

• Main Authors: LIU YACHAO, XU QIANG, GAO KUN, YAN JINQI, WANG YU, YU JIYUN, SONG WEIWEI, XIA YU, QIU CHUANGJUN, LI ZHONGMING
• Format: Patent
• Language: Chinese; English
• Published: 20.05.2022
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; VINEGAR; WINE

The invention discloses a fusion gene and a recombinant novel coronavirus high-efficiency immune tripod molecular DNA vaccine as well as a construction method and application thereof, and belongs to the technical field of biomedicine. The immune tripod molecular DNA vaccine ZD-nCor19 provided by the invention takes a novel coronavirus RBD protein, a 301-538aa segment of an S2 subunit and a 138-369aa segment of an N protein as target antigens, and specific immune synergistic molecules are introduced at proper positions, so that humoral immunity and cellular immunity can be efficiently induced at the same time; the ADE-related safety problem possibly generated by the full-length S protein and the full-length N protein can be avoided, the dual effects of prevention and treatment are achieved, and the vaccine can be used as a safe, efficient and stable vaccine variety for resisting novel coronavirus infection. 本发明公开了一种融合基因及一种重组新型冠状病毒高效免疫鼎分子DNA疫苗及其构建方法和应用，属于生物医学技术领域。本发明提供的免疫鼎分子DNA疫苗ZD-nCor19以新型冠状病毒RBD蛋白、S2亚基的301-5