Semen source identification and personal identification kit based on CpG-InDel/STR marker

• Main Authors: LI YIDAN, LI ZEQIN, ZHANG GENGQIAN, YAN JIANGWEI
• Format: Patent
• Language: Chinese; English
• Published: 13.05.2022
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses a seminal fluid source identification and personal identification kit based on a CpG-InDel/STR marker, which comprises 17 pairs of specific PCR (Polymerase Chain Reaction) primers with nucleotide sequences as shown in SEQ NO.1-34, and the 17 pairs of specific PCR primers are respectively and correspondingly used for specifically detecting that 17 seminal fluid specific CpG sites are combined with one or more InDel/STR linkage markers. According to the invention, through amplification and comparison of 17 semen specific CpG-InDel/STR markers and specific CpG indicator markers of other body fluids, body fluid sources in a mixed sample are detected, personal identification is carried out, individual typing of a semen donor is confirmed, and the method can be applied to forensic medicine identification of mixed seminal plaques. 本发明公开了一种基于CpG-InDel/STR标记的精液来源鉴定及个人识别试剂盒，包括有SEQ NO.1～34所示核苷酸序列的17对特异性PCR引物，分别对应用于特异性检测17个精液特异性CpG位点联合一个或多个InDel/STR的连锁标记。本发明通过扩增、比对17个精液特异性CpG-InDel/STR标记及其他体液的特异性C