Method for preparing polymer coupling body in one step without coupling agent

• Main Authors: JI XINGHU, ZHOU XINYUE, YI KEBING, HE ZHIKE
• Format: Patent
• Language: Chinese; English
• Published: 10.05.2022
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS BASED THEREON; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVINGCARBON-TO-CARBON UNSATURATED BONDS; MEASURING; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; ORGANIC MACROMOLECULAR COMPOUNDS; PEPTIDES; PHYSICS; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TESTING; THEIR PREPARATION OR CHEMICAL WORKING-UP; VINEGAR; WINE

The invention provides a method for preparing a polymer coupling body in one step without a coupling agent, which comprises the following steps: adding a to-be-coupled substance, a polymer monomer and an initiator into a buffer solution according to a certain proportion, and reacting at a certain temperature for a certain time to obtain a first product containing the polymer coupling body; wherein the to-be-coupled substance is selected from one or a combination of more of phenol derivatives, proteins and cells; the polymer monomer is selected from N-isopropylacrylamide, acrylamide or acrylic acid. Compared with the traditional coupling reaction, the method for preparing the polymer coupling body provided by the invention has the advantages of mild conditions, quick reaction, simplicity and convenience in operation, low cost and the like; meanwhile, the use of a coupling agent is avoided, so that the influence of the coupling agent on the physiological functions of proteins and cells and the physicochemical p