Primer combination and method for identifying biological source of chondroitin sulfate by using primer combination

• Main Authors: YANG SHENGPING, LAI ZHIHUA, DONG TINGTING, LUO FENG, BAI XUEDONG
• Format: Patent
• Language: Chinese; English
• Published: 06.05.2022
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses a primer combination and a method for identifying the biological source of chondroitin sulfate by using the primer combination. The primer combination comprises at least one primer pair, the primer pair comprises an upstream primer and a downstream primer, and the upstream primer is selected from one or more of nucleotide sequences as shown in SEQ ID NO: 1, SEQ ID NO: 3, SEQ ID NO: 5, SEQ ID NO: 7 and SEQ ID NO: 9; the downstream primer is selected from one or more of nucleotide sequences as shown in SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8 and SEQ ID NO: 10. The invention also discloses a kit containing the primer combination, and application of the primer combination and the kit in identifying the biological source of chondroitin sulfate. According to the method, chondroitin sulfate from sharks, chickens, pigs and cows in the market can be well identified, and particularly, the chondroitin sulfate sample with too low DNA content or serious damage can be subjected to bio