LAMP primer and kit for detecting mycoplasma

• Main Authors: ZHANG JIAN, WU YUMAN, KONG WEISHENG, CHEN ZHIYAN
• Format: Patent
• Language: Chinese; English
• Published: 29.04.2022
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention provides LAMP primers and a kit for detecting mycoplasma, and belongs to the technical field of gene engineering, the LAMP primers comprise an inner primer, an outer primer and a loop primer, and the nucleotide sequences of the primers are sequentially shown as SEQ ID No: 1-5. By adopting the LAMP primer provided by the invention, a detection result can be obtained within 50 minutes, and the sensitivity reaches 10 <-2 > ng/ul. 本发明提供了一种检测支原体的LAMP引物和试剂盒，属于基因工程技术领域，所述LAMP引物包括内引物、外引物和环引物，引物的核苷酸序列依次如SEQ ID No1～5所示。采用本发明提供的LAMP引物50min得到检测结果，灵敏度达到10-2ng/ul。