Preparation method and application of high-concentration agarose electrophoresis gel

• Main Authors: PENG CUNZHI, CHANG LILI, TONG ZHENG, XU BINGQIANG, WANG DAN, HUANG CHAO
• Format: Patent
• Language: Chinese; English
• Published: 26.04.2022
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention belongs to the technical field of biology, and relates to a preparation method and application of high-concentration agarose electrophoresis gel, a buffer solution and agarose are mixed to prepare an agarose solution, the agarose solution is dissolved in a steam environment under high-temperature and high-pressure conditions to obtain agarose sol, a glass plate glue pouring device is preheated and then injected into the agarose sol, a comb is inserted, and the agarose gel is obtained. And cooling and solidifying at room temperature to obtain the high-concentration agarose electrophoresis gel, and application of the high-concentration agarose electrophoresis gel in low-molecular-weight nucleic acid separation. The method is simple in process and convenient to operate, agarose is dissolved in a steam environment under high-temperature and high-pressure conditions to form uniform sol, the sol is filled in a high-temperature environment and cooled and solidified at room temperature to obtain the hig