Kit for inducing stem cells to differentiate into hepatocytes and application thereof

• Main Authors: WU HAIBIN, CHEN HONGLIN, TANG XIANGLIAN, DUAN YUYOU
• Format: Patent
• Language: Chinese; English
• Published: 26.04.2022
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention belongs to the technical field of biology, and discloses a kit for inducing stem cells to differentiate into hepatocytes and application of the kit. The kit comprises a first culture medium and a second culture medium, the first culture medium is a basic culture medium containing a GSK3beta inhibitor; the second culture medium is a basic culture medium containing Activin A; the final concentration of the GSK3beta inhibitor in the first culture medium is 6-12 [mu] M; the final concentration of the Activin A in the second culture medium is 1-10 ng/mL, and does not contain 10 ng/mL. According to the kit, a small molecule compound GSK3beta inhibitor and a low-concentration growth factor Activin A are combined and matched to replace high-concentration and expensive growth factors Activin A and Wnt3a, so that the cost is reduced, and meanwhile, the endoderm differentiation efficiency and the function, maturity and quality of hepatocytes can be improved. 本发明属于生物技术领域，公开了一种诱导干细胞分化为肝细胞的试剂盒及其应用。该试剂盒包含：第一培养