Ginkgo regeneration method based on leaf induction

• Main Authors: WANG JIE, ZHANG XIANXING, SANG YALIN, ZHOU YANGYAN
• Format: Patent
• Language: Chinese; English
• Published: 22.04.2022
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; NEW PLANTS OR PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES; TRAPPING

The invention discloses a culture medium composition for gingko regeneration. The culture medium composition comprises a gingko leaf induction callus culture medium, a gingko adventitious bud elongation culture medium and a gingko rooting culture medium, the ginkgo leaf callus induction culture medium comprises an MS culture medium, 4-8 g/L of agar, 25-32 g/L of cane sugar, 0.08-0.15 mg/L of IAA and 0.06-0.10 mg/L of IBA; the gingko adventitious bud elongation culture medium comprises an MS culture medium, 4-8 g/L of agar, 25-32 g/L of cane sugar, 0.25-0.40 mg/L of IBA and 0.06-0.12 mg/L of zeatin. The gingko rooting culture medium comprises a 1/2 MS culture medium, 4-8 g/L of agar, 25-32 g/L of cane sugar, 0.20-0.30 mg/L of IBA and 0.2-0.4 mg/L of NAA. The invention also discloses a gingko regeneration method using the culture medium composition. 本发明公开了一种用于银杏再生的培养基组合，包括银杏叶片诱导愈伤培养基、银杏不定芽伸长培养基和银杏生根培养基；银杏叶片诱导愈伤培养基包括MS培养基、4-8g/L琼脂、25-32g/L蔗糖、0.08-0.15mg/L IAA和0.06-0.10mg/L IBA；银杏不定芽伸长培养基包括MS培养基、4-8g/L琼脂、25-32g/L