Digital microscopic imaging system and microscopic examination method for biological sample

The invention discloses a digital microscopic imaging system for a biological sample, which comprises a digital microscope, an illumination module and a focusing mechanism, and is characterized by further comprising an automatic focusing calculation unit for controlling the focusing mechanism to adj...

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Bibliographic Details
Main Authors LYU XIAOHUA, HU QINGLEI, CHEN DUAN, ZENG SHAOQUN
Format Patent
LanguageChinese
English
Published 12.04.2022
Subjects
CALCULATING
COMPUTER SYSTEMS BASED ON SPECIFIC COMPUTATIONAL MODELS
COMPUTING
COUNTING
OPTICAL ELEMENTS, SYSTEMS, OR APPARATUS
OPTICS
PHYSICS
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Summary:The invention discloses a digital microscopic imaging system for a biological sample, which comprises a digital microscope, an illumination module and a focusing mechanism, and is characterized by further comprising an automatic focusing calculation unit for controlling the focusing mechanism to adjust the distance d between a sample surface and an object space focal plane of the digital microscope to be within a specified range, a digital microscope acquires a microscopic digital image of a sample, where n represents an object-side refractive index, lambda represents an illumination wavelength, and NA represents a numerical aperture of an objective lens of the digital microscope. The microscopic imaging system can be used for directly imaging a transparent or semitransparent sample which is not really dyed; the limitation caused by sample dyeing in a traditional microscopic examination method can be solved, the microscopic examination steps are simplified, and the microscopic examination time is shortened. M
Bibliography:Application Number: CN202111507652