Preparation method and application of chip with hydrophilic-super-hydrophobic composite interface

• Main Authors: ZENG ZHOUFANG, WANG YANDONG
• Format: Patent
• Language: Chinese; English
• Published: 12.04.2022
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention provides a preparation method and application of a chip with a hydrophilic-super-hydrophobic composite interface. The chip can be used for improving the crystallization uniformity of a sample and a matrix in MALDI (matrix-assisted laser desorption ionization) mass spectrometric detection and improving the reproducibility of mass spectrometric detection. The preparation method comprises the following steps: constructing a hydrophilic bare silicon area in the middle and a nano-structure super-hydrophobic area around a substrate on the substrate, dropwise adding a mixed solution of a matrix and a biological sample on the chip, and naturally airing at room temperature to obtain uniform matrix and sample co-crystallization particles. The chip disclosed by the invention can play a role in enriching a sample in mass spectrometry detection, and can also improve or even eliminate the influence caused by a coffee ring effect, improve co-crystallization of a matrix and the sample and improve the reproducib