Application of flow cytometer in cell counting and method

• Main Authors: WU CHUNBIN, CHENG YU, HUANG SHIANG, SHAO MENG, LIU LIYUAN, ZHANG JUAN, GUAN TIANYANG, LIANG CHAO, CHEN ZHONG, ZHOU XUE, WANG XIANFENG, NI HUALI
• Format: Patent
• Language: Chinese; English
• Published: 12.04.2022
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention relates to the technical field of biology, in particular to application and a method of a flow cytometer in cell counting, a liquid flow system of the flow cytometer is a liquid flow system for sampling by using pressure difference between a sample and sheath fluid, and the cell counting comprises the step of detecting the total cell number or cell concentration in a sample to be detected, the specimen to be detected is selected from peripheral blood or bone marrow or a suspension of a cell line. According to the cell counting method, the detection speed can be greatly increased, the detection accuracy is high, the sensitivity is high, the use amount of samples is small, a microscope and a cell counting instrument do not need to be additionally purchased, and cell counting and flow online detection can be completed by using one flow cytometer; meanwhile, long-time training on experimenters due to manual counting is omitted, serious dependence on special counting instruments and consumables and p