Protein, gene, recombinant vector, expression cassette, host and application

• Main Authors: GAO FEI, FAN YONGQIAN, JIAO JUAN, ZHAO MANCANG
• Format: Patent
• Language: Chinese; English
• Published: 12.04.2022
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention relates to the technical field of biology, in particular to a protein, a gene, a recombinant vector, an expression cassette, a host and application. Compared with the conventional glucose 6-phosphate isomerase, the protein provided by the invention has the advantages that the 521st amino acid is glutamic acid, the protein has the activity of the glucose 6-phosphate isomerase, and the enzyme activity is improved by at least 40% compared with the conventional glucose 6-phosphate isomerase. 本发明涉及生物技术领域，具体涉及一种蛋白、基因、重组载体、表达盒、宿主及用途。本发明提供的蛋白，相较于常规的6-磷酸葡萄糖异构酶，其第521位氨基酸为谷氨酸，具有6-磷酸葡萄糖异构酶活性，且酶活力相较于常规的6-磷酸葡萄糖异构酶提升了至少40%。