Solid culture medium, liquid culture medium and bifidobacterium longum high-density culture method

The invention relates to the field of bifidobacteria, and particularly discloses a solid culture medium, a liquid culture medium and a bifidobacterium longum high-density culture method. The solid culture medium comprises a moringa seed extract, yeast extract powder, glucose, peptone, anhydrous sodi...

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Bibliographic Details
Main Authors YAN LIQING, ZHOU GUOHUA, JIN ZHIPENG, ZHANG XIAODONG, LEE JIN-IK
Format Patent
LanguageChinese
English
Published 12.04.2022
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROCESSES USING MICROORGANISMS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:The invention relates to the field of bifidobacteria, and particularly discloses a solid culture medium, a liquid culture medium and a bifidobacterium longum high-density culture method. The solid culture medium comprises a moringa seed extract, yeast extract powder, glucose, peptone, anhydrous sodium acetate, triammonium citrate, dipotassium bicarbonate, magnesium sulfate, potassium carbonate, tween-80, agar and water. The liquid culture medium comprises fresh milk, beef powder, moringa seed extract, peptone, triammonium citrate, dipotassium bicarbonate, sodium chloride, potassium carbonate, tween-80, sodium alginate and water. The high-density culture method of the bifidobacterium longum comprises the following steps: firstly, inoculating a strain on the solid culture medium for activation; and transferring the strain into the liquid culture medium for further activation. According to the application, the moringa seed extract rich in nutrient substances is introduced into the culture medium, and the moringa
Bibliography:Application Number: CN202111668716