Preparation method and device of mesenchymal stem cell exosome

• Main Authors: OUYANG JIANHUA, MEI JIANXUN, HE WEIXIAN, WANG KAI
• Format: Patent
• Language: Chinese; English
• Published: 12.04.2022
• Subjects: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention provides a preparation method and device of mesenchymal stem cell exosomes, the preparation method of the mesenchymal stem cell exosomes is completed by using the preparation device of the mesenchymal stem cell exosomes, the preparation method of the mesenchymal stem cell exosomes comprises the following steps: 1) taking a gelatin aqueous solution, and preparing a gelatin microsphere crude product by using a double-emulsion method; dehydrating the gelatin microsphere crude product with absolute ethyl alcohol, and crosslinking in an absolute ethyl alcohol solution containing genipin to prepare a gelatin microcarrier; (2) putting a culture solution containing a gelatin microcarrier into a box body, inoculating the mesenchymal stem cells into the culture solution, carrying out stirring culture by using a preparation device of the mesenchymal stem cell exosome, and when the growth confluence degree of cells attached to the gelatin microcarrier is 80-90%, taking out the mesenchymal stem cell exosome,