Preparation method and device of mesenchymal stem cell exosome

The invention provides a preparation method and device of mesenchymal stem cell exosomes, the preparation method of the mesenchymal stem cell exosomes is completed by using the preparation device of the mesenchymal stem cell exosomes, the preparation method of the mesenchymal stem cell exosomes comp...

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Bibliographic Details
Main Authors OUYANG JIANHUA, MEI JIANXUN, HE WEIXIAN, WANG KAI
Format Patent
LanguageChinese
English
Published 12.04.2022
Subjects
APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:The invention provides a preparation method and device of mesenchymal stem cell exosomes, the preparation method of the mesenchymal stem cell exosomes is completed by using the preparation device of the mesenchymal stem cell exosomes, the preparation method of the mesenchymal stem cell exosomes comprises the following steps: 1) taking a gelatin aqueous solution, and preparing a gelatin microsphere crude product by using a double-emulsion method; dehydrating the gelatin microsphere crude product with absolute ethyl alcohol, and crosslinking in an absolute ethyl alcohol solution containing genipin to prepare a gelatin microcarrier; (2) putting a culture solution containing a gelatin microcarrier into a box body, inoculating the mesenchymal stem cells into the culture solution, carrying out stirring culture by using a preparation device of the mesenchymal stem cell exosome, and when the growth confluence degree of cells attached to the gelatin microcarrier is 80-90%, taking out the mesenchymal stem cell exosome,
Bibliography:Application Number: CN202111671565