Flunixin artificial antigen as well as preparation method and application thereof

• Main Authors: SHEN JIANZHONG, ZHANG SUXIA, WEN KAI, WANG ZHANHUI, SHI WEIMIN, YU XUEZHI, YU WENBO, MI JIAFEI
• Format: Patent
• Language: Chinese; English
• Published: 12.04.2022
• Subjects: CHEMISTRY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; METALLURGY; ORGANIC CHEMISTRY; PEPTIDES; PHYSICS; TESTING

The invention relates to a flunixin artificial antigen as well as a preparation method and application thereof. The flunixin artificial antigen provided by the invention is a compound as shown in a formula I, wherein K represents carrier protein. When the artificial antigen provided by the invention is used for immunizing animals, a high-sensitivity specific antibody can be prepared, and the method is simple, convenient and easy to implement. When the artificial antigen and the antibody prepared from the artificial antigen provided by the invention are applied to veterinary drug residue detection, rapid high-throughput detection on flunixin at a trace level can be realized. 本发明涉及一种氟尼辛人工抗原及其制备方法与应用。本发明提供的氟尼辛人工抗原为式Ⅰ所示化合物，其中K表示载体蛋白。使用本发明提供的人工抗原免疫动物，可制备得到高灵敏度的特异性抗体，方法简便、易行。将本发明提供的人工抗原及其制备的抗体应用到兽药残留检测中，可实现在痕量水平上对氟尼辛进行快速高通量检测。