Method for constructing colorectal cancer PDX model

The invention is applicable to the technical field of biological science, and provides a colorectal cancer PDX model construction method which comprises the following steps: S1, collecting a human colorectal cancer sample, and transporting and storing the human colorectal cancer sample; s2, culturin...

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Bibliographic Details
Main Authors YANG XIU, LIU JIA, CHENG XIANSHUO, WANG TINGHUA, ZHANG LANCHUN, XIONG LIULIN
Format Patent
LanguageChinese
English
Published 12.04.2022
Subjects
AGRICULTURE
ANIMAL HUSBANDRY
CARE OF BIRDS, FISHES, INSECTS
FISHING
FORESTRY
HUMAN NECESSITIES
HUNTING
NEW BREEDS OF ANIMALS
REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR
TRAPPING
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Summary:The invention is applicable to the technical field of biological science, and provides a colorectal cancer PDX model construction method which comprises the following steps: S1, collecting a human colorectal cancer sample, and transporting and storing the human colorectal cancer sample; s2, culturing human colorectal cancer primary cells, and carrying out subculture; s3, performing cell liquid change and subculture cryopreservation, and counting the cells when the human colorectal cancer primary cells are subjected to cryopreservation; s4, cell identification: culturing human colorectal cancer primary cells, and performing immunofluorescence identification and HE staining identification on the cultured cells; s5, carrying out cell inoculation; s6, performing human colorectal cancer PDX model construction observation and index detection.The human colorectal cancer primary cell culture method is optimized, the colorectal cancer PDX model is constructed, different anti-tumor drugs are selected for colorectal can
Bibliography:Application Number: CN202210002279