Lung cancer organoid culture solution and culture reagent combination and culture method thereof

• Main Authors: LIU JIANCHUANG, XIAO RONGRONG, LI PEIWEN, SUN YANTING, ZHANG XIAOHUI, ZHOU YU
• Format: Patent
• Language: Chinese; English
• Published: 08.04.2022
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention relates to the technical field of biological tissue engineering, and discloses a lung cancer organoid culture solution and a culture reagent combination and a culture method thereof, and the lung cancer organoid culture solution comprises a complete medium and an autogenous condition medium; the complete culture medium comprises a conditioned culture medium, a basic culture medium, compound antibiotics and growth factors; the autogenous condition culture medium is obtained by culturing fiber cells extracted from lung cancer tissues by using a tumor-associated fibroblast culture medium. All the components of the culture solution have a synergistic effect, so that the formation and growth of lung cancer organoid cells are accelerated, and the culture speed is increased while the cell harvesting number is increased; and the in-vitro cultured organoid is more bionic, can be suitable for personalized culture of the lung cancer organoid, and can maintain the physical structure and pathological charact