Kit for detecting CD16A gene polymorphism as well as application and use method thereof

• Main Authors: WEI DONGKAI, WEN BIN, WANG XI
• Format: Patent
• Language: Chinese; English
• Published: 05.04.2022
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses a kit for detecting CD16A gene polymorphism as well as application and a use method of the kit. The kit comprises a first primer pair for amplifying a CD16A gene sequence, a second primer pair for detecting the rs396991 site polymorphism of a CD16A gene and a fluorescent probe, the first primer pair is used for amplifying a CD16A gene sequence; the second primer pair is used for detecting the rs396991 site polymorphism of the CD16A gene; the fluorescent probe comprises a probe for detecting the rs396991 site polymorphism of the CD16A gene, the probe comprises a first probe 158F-probe: 5 '-FAM-TTACTCCAAAAAGCCCCCTG-MGB-3' and a second probe 158V-probe: 5 '-HEX-TTACTCCAAAAAGCCCCCTG-MGB-3', and the fluorescent probe has the advantages of being simple and convenient to operate, high in accuracy, good in specificity, low in cost and the like. 本发明公开了一种检测CD16A基因多态性的试剂盒及其应用和使用方法，试剂盒包括扩增CD16A基因序列的第一引物对和检测CD16A基因rs396991位点多态性的第二引物对和荧光探针；扩增CD16A基因序列的第一引物对；检测CD16A基因rs396991位点多态性的第二引物对；荧光探针包括检测CD16A