Freeze-drying protective agent and application thereof

• Main Authors: LI SHUGUANG, DUAN YANXU, CHEN LIANG, SUN YUN, ZHAO XINYING
• Format: Patent
• Language: Chinese; English
• Published: 05.04.2022
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; VINEGAR; WINE

The invention discloses a freeze-drying protective agent and application thereof. The freeze-drying protective agent comprises the following components in parts by weight: 5-20 parts of stachyose; 10 to 30 parts of maltodextrin; 0.01 to 0.2 part of gelatin; 0.1 to 5 parts of a surfactant; and 100 parts of water. The freeze-drying protective agent is applied to preparation of freeze-drying microspheres of a real-time fluorescent quantitative PCR reaction solution. The freeze-drying microspheres prepared by adopting the freeze-drying protective agent disclosed by the invention have the characteristics of regular form, stable structure, smooth and round surface, low hygroscopicity and the like. 本发明公开了一种冻干保护剂及其应用。冻干保护剂各成分的重量份数为：水苏糖5~20；麦芽糊精10~30；明胶0.01~0.2；表面活性剂0.1~5；水100。该冻干保护剂应用于制备实时荧光定量PCR反应液的冻干微球。采用本发明的冻干保护剂制备的冻干微球具有形态规则，结构稳定，表面光滑圆润，吸湿性小等特点。