Purple leaf rice two-line sterile line and breeding method and application thereof

• Main Authors: LIU KAIQIANG, DENG GUOFU, QING DONGJIN, LIANG HAIFU, GAO JU, WU HAO, GAO LIJUN, ZHOU WEIYONG, DAI GAOXING, CHEN WEIWEI, PAN YINGHUA, LI JINGCHENG, HUANG JUAN, SU SHUQUAN
• Format: Patent
• Language: Chinese; English
• Published: 01.04.2022
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NEW PLANTS OR PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TRAPPING; VINEGAR; WINE

The invention belongs to the technical field of molecular biological breeding, and discloses a purple leaf rice two-line sterile line and a breeding method and application thereof. The two-line sterile line of the purple-leaf rice is bred under the assistance of molecular markers of purple-leaf character genes DFR and plr4 of the rice, the two-line sterile line of the purple-leaf rice, a hybrid F1 of green-leaf rice and a plant generated by selfing of the hybrid F1 have leaf color difference in the seedling stage and even in the whole growth and development process, the hybrid F1 plant shows green leaves, the plant generated by a selfing line shows purple leaves, and the purple-leaf character genes DFR and plr4 have the advantages that the purple-leaf character genes DFR and plr4 can be obtained; the method can be applied to seed purity identification, auxiliary impurity removal or automatic false discharge in hybrid rice breeding. According to the method, the breeding period can be shortened, the breeding ac