Indian cochlea, preparation method thereof and application of Indian cochlea in production of vitamin B12

• Main Authors: LIU QIAN, YU CHUNHE, WANG LIANGMING, GAO XIANXING, CHAI XUE, ZHANG SHUANGSHUANG, ZHANG CUIXUAN
• Format: Patent
• Language: Chinese; English
• Published: 25.03.2022
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses a coriolus versicolor, a preparation method of the coriolus versicolor and application of the coriolus versicolor in production of vitamin B12. The coriolus versicolor is named as the coriolus versicolor Nhu HG-22, the preservation number of the coriolus versicolor is CGMCC (China General Microbiological Culture Collection Center) No.23306, and the preservation date of the coriolus versicolor is August 27, 2021. The invention provides a mutant strain which is obtained by performing ultraviolet mutagenesis and lithium chloride mutagenesis treatment on starting bacteria, namely cochlea indica Nhu 015-9, the maximum concentration of the mutant strain is achieved after the mutant strain is fermented for about 144 hours, the concentration of the mutant strain is improved by 16.4% compared with that of the starting bacteria at the same time, the logarithmic phase of the mutant strain is shortened by 2 days compared with that of the starting bacteria, and the yield of the mutant strain is gre